dextran hydrodynamic radius

Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. GYA primarily conducted research. International Journal of Biological Macromolecules, 23, 135-141. 2019;9(3-S):9726. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Rheology of dextran solutions. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Critical parameters for gel permeation 1984;17(6):11703. Microporation was performed 2 times on each sample. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Human skins (Full-thickness human skin) were supplied by HansBiomed. ; Hogan, M.P. American Journal of Food Science and Technology 2013, 1(3), 60-66. Chemical penetration enhancers for transdermal drug delivery systems. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Optical properties of dextran in solution and in films. Harding, Stephen E. (2005). The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Search In fact, short chains do contain minor branching points. 2012;9(2):14754. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. The micropore formed at the SC through to the epidermis, enabling drug delivery. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Epub 2021 Feb 9. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Microporation techniques for enhanced delivery of therapeutic agents. The slope is a value and intercept is equal to the ln kMH value [47]. Ioan, C.E. 1905/05) for their financial support. Macromolecules. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Walkenhorst, S.; Olivier, R. (2002). The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. (2003). The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Thus, it is a common practice to express the frictional coefficient of rigid structures as. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Pure Appl. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Int. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Pharm Res. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Proc. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. 151-166. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Durand, A. ; Wood, P.J. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Learn More. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Dependence on Concentration. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. After 24h, the cumulative permeation of FITC-dextran M.W. Probiotics Antimicrob Proteins. The enhanced permeability through micropores was confirmed by Franz diffusion cell. The solution was measured using an Anton Paar DMA35N densitometer. ; Rani, K.Y. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. ; Bataille, B. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Kasaai, Mohammad R. (2007). ; Talsma, H.; Hennink, W.E. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). ; Yevlampieva N.P. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Macromolecules, 31, 7712-7716. The hydrodynamic 57 radius of the dextranase is 7 (2008). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. B 113, 8020-8025. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Dextran. WebR H Hydrodynamic radius [m] The basic DLS setup. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Increased TEWL values are evidence of micropores formation. where tr is the column retention time in minutes (2 of 0.9792). 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. 2015;32(5):170413. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Soc., 16, 3A, 426-433. Differences were considered statistically significant at p <0.05. Google Scholar. Carbohydrate Polymers, 79, 380-390. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Dextran Fractions. Corp. (Seoul, Korea). Eng. Carbohydrate Research, 160, 205-214. Determination of intrinsic viscosities of macromolecules and nanoparticles. The authors declare that they have no competing interests. Hydrodynamic Properties of Tragacanthin. (1999). 2010;4(1):117. Bethesda, MD 20894, Web Policies Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Ind. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Pharm Res. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. European Polymer Journal, 42, 1086-1092. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. The genus Weissella: taxonomy, ecology and biotechnological potential. Webwhittier union high school district superintendent. (2008). The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Progress in Biophysical Molecules Biological, 68, 207-262. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Carbohydrate Polymers, 68, 477-488. The Mark-Houwink. Curley SA. J Pharm Sci. Format), Citation-(BibTeX Epub 2022 Feb 2. De Belder, A.N. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Journal of Physical Chemistry. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Webdextran hydrodynamic radius. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). We observe that this maximum radius is below Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Formul. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Advanced Drug Delivery Reviews, 60, 1650-1662. Journal of American Chemical Society, 76 (20), 5041-5052. Charge: Anionic. J Cosmet Laser Ther. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Figure5 shows the cumulative amount of the FITC-dextran (M.W. National Library of Medicine Stenekes, R.J.H. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). pp13-21. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. GPC Symp. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. 2013;3(5):3588605. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. osmotic pressure or light scattering). Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Physicochemical studies of oligodextran. ; Prasad, D.H.L. (2013). WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. Formation of dextran hydrogels by crystallization. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Hindered diffusion of dextran and ficoll in microporous membranes. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Hydrodynamic radii ( RH) were determined by dynamic light scattering. Carbohydrate Research, 340, 811-826. 8600 Rockville Pike Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. 2008;28(5):64557. Nair SS. Drug Deliv. Epub 2017 Jun 1. Table 2 compares the properties of nanoparticles coated with one or other polymer. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Keywords: FITC dextran; intestinal permeability; transit time. (1954). TEWL measurements were taken 30min after microporation of the human and pig skin. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Carbohydrate Polymers 88 1440-1444. 2013;43(4):287303. Dilute Solution. (2006). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , For Research Use Only. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Progress in Polymer Science, 30, 38-70. Manage cookies/Do not sell my data we use in the preference centre. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. 20K groups (n=3, *: p<0.05, **: p<0.01). The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. ; Grishchenko, A.E. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Carbohydrate Polymers, 78, 902-907. Journal Non-Newtonian Fluid Mech., 97, 295-301. Webof overall macromolecular geometry and hydrodynamic draining. ; van Nostrum, C.F. (2012). Degrees of long-chain branching in dextrans. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior.

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